I took a swab covered in broth and swiped it against the Braille label for the autoclave room. I then used the swab to make streaks on the bottom of the Petri dish to spread the bacteria. I put the top part back on the dish and flipped it upside-down before putting it in the incubator.
September 8th, 2011
Upon inspecting my Petri dish the next day, I found three types of bacteria growing in it. The first type was circular, yellow, and flat with entire edges. The second type was rhizoid in shape, was too dark in color to determine what color it actually was, looked flat and had filamentous ends. The third type was white, had an irregular shape with undulating edges, and seemed to be ingrowing. I decided to isolate the white colony from the rest by rubbing the contents off the original plate with an inoculating loop and spreading it on a new Petri dish and flipping it upside-down when I was done. I sterilized the loop and put my Petri dishes in their designated areas, the new one in the incubator and the original in the refrigerator.
